Question: up regulated genes based on fold change
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gravatar for Learner
4.5 years ago by
Learner 230
Learner 230 wrote:

I have 50 different individuals in three biological replicates (50 x 3=150) Then I calculate the fold change using this

sample/control 
replicate/control
replicate/ control

The second replicate is obtained in a shift time (I want to also get the idea on the time influence in my experiment) The third replicate is obtained in a bit more shift time

Now I would like to obtain which genes are up regulated and which one is down regulated.

My own idea is to sort the data based on the three expression. then select the top of the list as up and those that have a very low expression as down regulated. is it a good approach ?

gene • 1.1k views
ADD COMMENTlink modified 4.5 years ago by WouterDeCoster44k • written 4.5 years ago by Learner 230
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gravatar for WouterDeCoster
4.5 years ago by
Belgium
WouterDeCoster44k wrote:

I would suggest you have a look at the R/Bioconductor package limma: https://bioconductor.org/packages/release/bioc/html/limma.html

ADD COMMENTlink written 4.5 years ago by WouterDeCoster44k
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