Dear all,
I need to use GTF annotation file for my RNASeq project. I downloaded GTF for human from ensembl, but it did not work well when I used it in Cufflinks. In fact all FPKMs were recorded as zero.
Some one advised me to use gtf annotation from iGenomes.
Unfortunately I faced with difficulty in downloading it from iGenomes.
Can anyone tell me what should I do?
can I download this file from any other sources? or can anyone send it to me?
I appreciate any help in advance Nazanin
What goes wrong when trying to download?
Thanks for your response. I could get it from Galaxy.