Question: ncRNA analysis RNA Seq
0
gravatar for clueless
3.3 years ago by
clueless0
clueless0 wrote:

I am new in the RNA Seq/R-script world and need help with some data I got from the RNA seq analysis. I would like to know how to analyze my raw counts files in respect of ncRNAs (which are overall expressed, differential expression after treatment)? I know that I will "miss" a large portion of ncRNAs due to enrichment of poly-A RNA but I have quite unique samples and would like to give it a try. Can someone provide me with an R script that I can use? Please note I am an absolute R-beginner.

rna-seq R • 1.0k views
ADD COMMENTlink written 3.3 years ago by clueless0
1

While you may find examples of pre-made R scripts to do DE analysis you may want to go through the original tutorial by Love et al here.

ADD REPLYlink written 3.3 years ago by genomax70k
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