Question: TF and Promoter
0
gravatar for Azhar
2.8 years ago by
Azhar40
China
Azhar40 wrote:

I have a novel TF binding site related to my study to important gene promoter so i want to confirm that but i don,t have those samples so what kind of molecular techniques i can use?

chip-seq • 843 views
ADD COMMENTlink modified 2.8 years ago by Shicheng Guo7.4k • written 2.8 years ago by Azhar40
1
gravatar for Shicheng Guo
2.8 years ago by
Shicheng Guo7.4k
Shicheng Guo7.4k wrote:

Download TF and TFBS ChIP-seq data from UCSC:

http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeRegTfbsClustered/

And then try to find the cell type you want to validate and therefore you can get what you want to validate.

ADD COMMENTlink written 2.8 years ago by Shicheng Guo7.4k

I have chip seq data for a kind of human tissue , what I already visualized my peak file from ucsc encode data so i ask what should i do experimentally to vaildate

ADD REPLYlink written 2.7 years ago by Azhar40
1

clone the peak region into a report system (vector) and check the fluorescence signal.

ADD REPLYlink written 2.7 years ago by Shicheng Guo7.4k
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