Question: tools for gene assembly and quality check for sanger sequencing
0
gravatar for lait
3.5 years ago by
lait140
lait140 wrote:

Hi,

Can anyone advise me please, what is the best tool to use to perform gene assembly after performing sanger sequencing? we aim to sequence around 7 genes using sanger sequencing.

and one more question, is there another tool to check the quality of the fragments before assembly ? (like the case of fastqc in NGS).

many thanks

ADD COMMENTlink modified 3.5 years ago by ismaeilmohamed30410 • written 3.5 years ago by lait140
1
gravatar for ismaeilmohamed304
3.5 years ago by
ismaeilmohamed30410 wrote:

http://www.dnabaser.com/

ADD COMMENTlink written 3.5 years ago by ismaeilmohamed30410

do you know about any free tools ?

ADD REPLYlink written 3.5 years ago by lait140
0
gravatar for Benn
3.5 years ago by
Benn7.9k
Netherlands
Benn7.9k wrote:

Did you try Phred and Phrap?

http://www.phrap.org/phredphrapconsed.html

ADD COMMENTlink written 3.5 years ago by Benn7.9k
0
gravatar for ismaeilmohamed304
3.5 years ago by
ismaeilmohamed30410 wrote:

use dna basser it's for free up to 30 days

ADD COMMENTlink written 3.5 years ago by ismaeilmohamed30410
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