tools for gene assembly and quality check for sanger sequencing
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5.4 years ago
lait ▴ 160

Hi,

Can anyone advise me please, what is the best tool to use to perform gene assembly after performing sanger sequencing? we aim to sequence around 7 genes using sanger sequencing.

and one more question, is there another tool to check the quality of the fragments before assembly ? (like the case of fastqc in NGS).

many thanks

sanger sequencing fastq gene Assembly • 1.6k views
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do you know about any free tools ?

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5.4 years ago
Benn 8.2k

Did you try Phred and Phrap?

http://www.phrap.org/phredphrapconsed.html

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5.4 years ago

use dna basser it's for free up to 30 days

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