Question: Problems about NGStoolkit
0
gravatar for 543192933
4.8 years ago by
5431929330
5431929330 wrote:

I have used IlluQC.pl to perform quality control for my Illumina resequece data, but it continuously out put an error message : Modification of non-creatable array value attempted subscript -2 at IlluQC.pl line 1030 <f2> line 5445196. and then the procedure shut down. I use the paramater : perl IlluQC.pl -pe R01_1.fq R01_2.fq 2 A -p 4. What should I do? It already drove me mad, can any one help me?

sequencing • 910 views
ADD COMMENTlink modified 16 months ago by Biostar ♦♦ 20 • written 4.8 years ago by 5431929330

While someone familiar with the specific program you are referring to responds you may want to switch gears and try FastQC out.

ADD REPLYlink written 4.8 years ago by GenoMax96k

I have put the answer in the wrong place, thank you for your advise

ADD REPLYlink written 4.8 years ago by 5431929330

I'll add a plug for fastqp as well.

ADD REPLYlink written 4.8 years ago by Matt Shirley9.5k

Thanks! @Matt: Does the output include all those graphs in a single/standalone file like FastQC?

ADD REPLYlink written 4.8 years ago by GenoMax96k

Not an HTML report, but a zip file...

ADD REPLYlink written 4.8 years ago by Matt Shirley9.5k
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