Question: TopHat and Fastq with different Lane numbers
0
gravatar for kanwarjag
4.3 years ago by
kanwarjag1.0k
United States
kanwarjag1.0k wrote:

I have a very naive question will TopHat handle successfully in default conditions FastQ files merged from different lanes. I get a generic error TopHat failed. What will be best approach align Fastq fro different lanes separately then merge Bam?

Any suggestion pleas

rna-seq tophat • 1.1k views
ADD COMMENTlink modified 4.3 years ago by WouterDeCoster44k • written 4.3 years ago by kanwarjag1.0k
0
gravatar for WouterDeCoster
4.3 years ago by
Belgium
WouterDeCoster44k wrote:

A suggestion to narrow down the origin of your error would be to use Tophat on just one of the fastq files and see if the error reproduces or not.

ADD COMMENTlink written 4.3 years ago by WouterDeCoster44k
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