Hi all,
I recently installed the cutadapt with the: pip install --user --upgrade cut adapt I try to run the mode 3 of MaGeCK where I need to trim the 5'adapter seq for the fastq file ERR376998
The problem is that I do get the message:
bash: cutadapt: command not found
,when I try to run the order :
cutadapt -g CTTGTGGAAAGGACGAAACACCG -o output.fastq ERR376998.fastq
Any ideas why? Thank you very much!
Dear genomax2, thanx much! But still the same problem
The which cutadapt does not produce anything. Entered export PATH=/path_to_cutadapt_dir/:$PATH and again had the same result: -bash: cutadapt: command not found
I type echo $PATH and the path was changed /path_to_cutadapt_dir/:/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/bin
Any ideas?
Sorry if I was not clear. /
path_to_cutadapt_dir
needs to be replaced with a real directory on your machine.Can you see if
find ~ -name "cutadapt"
produces a local directory listing (under your $HOME)? If that says /home/Pigasos83/.local/bin/cutadapt
then (as an example) you would doexport PATH=/home/Pigasos83/.local/bin/:$PATH
thank you much! it worked :)
Perfect. Thanks for the confirmation.
Good luck with the rest of your work. Please upvote the correct answer here to show your appreciation :-)
Thanks for the heads up Wouter: Here I copy paste the correct commands: which cutadapt # to identify if the cutadapt is installed in your path. If it does not produce anything then the location where cutadapt was installed is not in your $PATH
find ~ -name "cutadapt" # produces a local directory listing (under your $HOME)?
export PATH=/home/Pigasos83/bin/:$PATH # exports the cutadapt on my $PATH
cutadapt -g CTTGTGGAAAGGACGAAACACCG -o output.fastq ERR376998.fastq # works!!!
Enjoy!