Entering edit mode
7.9 years ago
frida.danielsson
▴
50
Hi,
I want to use tophat for aligning paired-end reads from RNA-seq but I have had a lot of troubles with the program failing to report the output (seems like a common problem, several posts out there from people encountering the same issue), however I now managed to get around that problem by removing the --no-discordant flag, but my question is, how much does that decrease the quality of my output?
Frida
