Question: Is there a standard yaml file to describe Illumina runs: sample_name, barcode, lane, flowcell?
gravatar for 14134125465346445
2.1 years ago by
United Kingdom
141341254653464453.4k wrote:

Is there a format to describe sample names and their associated flowcell(s), lane(s) and barcode(s) from Illumina sequencing experiments?

The Illumina documentation describes the following notation for multiplex and non-multiplexed runs:

Illumina FASTQ files use the following naming scheme:
<sample name>_<barcode sequence>_L<lane (0-padded to 3 digits)>_R<read number>_<set number (0-padded to 3 digits>.fastq.gz
For example, the following is a valid FASTQ file name:
In the case of non-multiplexed runs, <sample name> will be replaced with the lane numbers (lane1, lane2, ..., lane8) and <barcode sequence> will be replaced with "NoIndex".

And I have seen bcbio has some code and example yaml files to describe some of this, and it seems scilifelab has adopted it:

What I am looking for is a standard or something close to a standard that people have adopted for this.

Does anything like this exist? Is Common Workflow Language CWL dealing with this? Galaxy? Genologics?

barcode lane flowcell illumina run • 898 views
ADD COMMENTlink modified 2.1 years ago by Pierre Lindenbaum108k • written 2.1 years ago by 141341254653464453.4k
gravatar for Pierre Lindenbaum
2.1 years ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum108k wrote:

Illumina CASAVA generates some XML files after demultiplexing:

  <Software Version="CASAVA-1.8.2" CmdAndArgs="...">
  <FlowcellInfo ID="C3FGGACXX" Operator="x" Recipe="" Desc="">
    <Lane Number="1">
      <Sample ProjectId="P1" Control="N" Index="CTTGTA" SampleId="S1"  />
      <Sample ProjectId="P2" Control="N" Index="GCCAAT" SampleId="S1" />
ADD COMMENTlink written 2.1 years ago by Pierre Lindenbaum108k
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