Question: GTAK VariantsToTable only generates header?
0
gravatar for epigene
3.4 years ago by
epigene490
United States
epigene490 wrote:

I wanted to run GATK VariantsToTable on a vcf file but somehow my command finished within 10 seconds and output a file with the header only.. so something must be wrong and I could't see where the issue comes from. Did anyone run into the same issue and know how to solve it?

I could only find a post that had similar issue but no direct answer..

http://gatkforums.broadinstitute.org/gatk/discussion/5260/variantstotable-for-multi-sample-vcfs

Here is the command line and running log info.

 java -jar ~/localbin/GenomeAnalysisTK.jar -R ~/genomes/hg38.fa -T VariantsToTable -V test.hg38.WGS.sort.vcf.gz -F CHROM -F POS -F ID -F REF -F ALT -o test.hg38.WGS.sort.vcf_table.txt
    INFO  22:47:28,527 HelpFormatter - --------------------------------------------------------------------------------
    INFO  22:47:28,531 HelpFormatter - The Genome Analysis Toolkit (GATK) v3.3-0-g37228af, Compiled 2014/10/24 01:07:22
    INFO  22:47:28,531 HelpFormatter - Copyright (c) 2010 The Broad Institute
    INFO  22:47:28,531 HelpFormatter - For support and documentation go to http://www.broadinstitute.org/gatk
    INFO  22:47:28,537 HelpFormatter - Program Args: -R ~/genomes/hg38.fa -T VariantsToTable -V test.hg38.WGS.sort.vcf.gz -F CHROM -F POS -F ID -F REF -F ALT -o test.hg38.WGS.sort.vcf_table.txt
    INFO  22:47:28,543 HelpFormatter - Executing as xx on Linux 2.6.18-164.6.1.el5 amd64; Java HotSpot(TM) 64-Bit Server VM 1.7.0-b147.
    INFO  22:47:28,543 HelpFormatter - Date/Time: 2016/06/16 22:47:28
    INFO  22:47:28,544 HelpFormatter - --------------------------------------------------------------------------------
    INFO  22:47:28,544 HelpFormatter - --------------------------------------------------------------------------------
    INFO  22:47:32,236 GenomeAnalysisEngine - Strictness is SILENT
    INFO  22:47:35,623 GenomeAnalysisEngine - Downsampling Settings: Method: BY_SAMPLE, Target Coverage: 1000
    WARN  22:47:37,344 IndexDictionaryUtils - Track variant doesn't have a sequence dictionary built in, skipping dictionary validation
    INFO  22:47:37,450 GenomeAnalysisEngine - Preparing for traversal
    INFO  22:47:37,480 GenomeAnalysisEngine - Done preparing for traversal
    INFO  22:47:37,481 ProgressMeter - [INITIALIZATION COMPLETE; STARTING PROCESSING]
    INFO  22:47:37,482 ProgressMeter -                 | processed |    time |    per 1M |           |   total | remaining
    INFO  22:47:37,483 ProgressMeter -        Location |     sites | elapsed |     sites | completed | runtime |   runtime
    INFO  22:47:38,168 ProgressMeter -            done         0.0     0.0 s       7.9 d      100.0%     0.0 s       0.0 s
    INFO  22:47:38,169 ProgressMeter - Total runtime 0.69 secs, 0.01 min, 0.00 hours
    INFO  22:47:39,534 GATKRunReport - Uploaded run statistics report to AWS S3
variantstotable gatk • 1.7k views
ADD COMMENTlink written 3.4 years ago by epigene490

Does this still happen if you pass an uncompressed VCF file through?

ADD REPLYlink written 3.4 years ago by andrew.j.skelton735.8k

haven't tried that, but i have a huge vcf file so zip and unzip it will be a headache.. actually i run the same command on another zipped vcf file and it run through. so i'm trying to troubleshoot what went wrong with vcf files. so you can run it with zipped vcf without a problem for sure.

ADD REPLYlink written 3.4 years ago by epigene490
1

You can, but there have been reports of instability. Decompressing won't take that long either, even if it's really big. Look into Pigz (unix utility), that can perform compression / decompression operations in parallel.

ADD REPLYlink written 3.4 years ago by andrew.j.skelton735.8k

i see. will look it up, thanks

ADD REPLYlink written 3.4 years ago by epigene490
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1259 users visited in the last hour