I have had successfully run Trinity for de novo assembly on my server, but recently ran in to this error:
Error, cannot locate file: heat_R1_P.fastq.gz at /home/program/trinityrnaseq-2.2.0/Trinity line 2173. main::create_full_path('ARRAY(0x282ac10)', 1) called at /home/program/trinityrnaseq-2.2.0/Trinity line 1109
my command line is:
Trinity --seqType fq --max_memory 10G --left ctl_R1_P.fastq.gz,heat_R1_P.fastq.gz --right ctl_R2_P.fastq.gz,heat_R2_P.fastq.gz --bypass_java_version_check --CPU 6 --no_version_check
Trinity version: v2.1.1
I have test /home/program/trinityrnaseq-2.2.0/sample_data/test_Trinity_Assembly/runMe.sh and it ran success. How do I fix this error?
Thanks for any suggestion