How can I find the critical genes for the pathway targeted by my miRNAs?

Question

[lol]

Hi,

Using mirpath v2, I choose 4 miRNAs and I click on a pathway. How can I find the critical genes for the pathway targeted by my miRNAs? What means "critical genes"?

Answer 1

[natasha.sernova]

See these papers:

My understanding of 'critical genes' is as those genes, that are obligatory for the pathway to function as it was supposed to. In many cases these are regulatory genes - they may make a pathway be active and support the activity, or decrease the activity and/or suppress it.

In this article:" A pathway analysis is then often performed in order to generate hypotheses about the relevant biological functions controlled by the miRNA signature. Here we show that the method widely used in scientific literature to identify these pathways is biased and leads to inaccurate results."

Pathway analysis from lists of microRNAs: common pitfalls and alternative strategy

http://nar.oxfordjournals.org/content/early/2015/03/23/nar.gkv249.full

In this article its authors wrote: " We present an overview of the major classes of miRNA tools and databases and discuss critical issues related to their selection. "

Bioinformatic tools for microRNA dissection

http://nar.oxfordjournals.org/content/early/2015/11/16/nar.gkv1221.full

Probing-directed identification of novel structured RNAs - it's not micro, sorry

http://www.tandfonline.com.sci-hub.cc/doi/abs/10.1080/15476286.2015.1132140

I think your question is close to these posts:

How To Build A Network Of Mirna And Their Target Genes?

How to map miRNA to transcripts of targeting genes ?

difference between mirna target and target mimics

Mirna-Target Gene Network

How Can I Find Target Genes Of A Transcription Factor?

how to know the gene name for a miRNA and mRNA ? Where is the database ?