I'm using the Galaxy interface to analyse RNAseq data. I've successfully aligned using the default reference genome, then counted using the gtf file downloaded from UCSC, then done the differential expression analysis with DeSeq2, which gives good results. I wanted to compare against the ensemble reference genome, but am struggling. I downloaded the DNA FASTA from here - http://www.ensembl.org/info/data/ftp/index.html - but can't get TopHat to align with it. Any help would be much appreciated
Question: (Closed) TopHat aligning with ensembl in Galaxy
2.9 years ago by
michael.flower.14 • 0 wrote:
ADD COMMENT • link •
Please log in to add an answer.
The thread is closed. No new answers may be added.
Powered by Biostar version 2.3.0
Traffic: 1881 users visited in the last hour