Question: (Closed) TopHat aligning with ensembl in Galaxy
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gravatar for michael.flower.14
2.9 years ago by
United Kingdom
michael.flower.140 wrote:

I'm using the Galaxy interface to analyse RNAseq data. I've successfully aligned using the default reference genome, then counted using the gtf file downloaded from UCSC, then done the differential expression analysis with DeSeq2, which gives good results. I wanted to compare against the ensemble reference genome, but am struggling. I downloaded the DNA FASTA from here - http://www.ensembl.org/info/data/ftp/index.html - but can't get TopHat to align with it. Any help would be much appreciated

ADD COMMENTlink written 2.9 years ago by michael.flower.140

Hello michael.flower.14!

We believe that this post does not fit the main topic of this site.

Please ask https://biostar.usegalaxy.org/

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 2.9 years ago by RamRS21k
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