Question

What is the illumina sequencing error rate?

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7.8 years ago

Sama ▴ 60

I want to simulate Illumina short reads to investigate performance of my algorithm. I need to know what the sequencing error rate of Illumina is. I also need to have the reference of this information to be able to cite it.

sequencing illumina error • 19k views

ADD COMMENT • link updated 7.8 years ago by Biostar 20 • written 7.8 years ago by Sama ▴ 60

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There are many existing NGS data simulation programs: NGS reads simulation

ADD REPLY • link 7.8 years ago by GenoMax 141k

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Actually I am using one of the existing softwares I just wanted to have a reference for the error rate.

ADD REPLY • link 7.8 years ago by Sama ▴ 60

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It depends on the platform, chemistry, read length, and experiment; there's no single Illumina error rate. It can range from under 0.1% to over 10%.

ADD REPLY • link 7.8 years ago by Brian Bushnell 20k

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As well as one the genomic context...

ADD REPLY • link 7.8 years ago by WouterDeCoster 47k

score 3 · Answer 1 · 2016-06-21

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7.8 years ago

GenoMax 141k

See this table: http://www.molecularecologist.com/next-gen-table-3c-2014/

ADD COMMENT • link 7.8 years ago by GenoMax 141k

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Thank you so much. I could find it in this paper: http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0998.2011.03024.x/full

ADD REPLY • link 7.8 years ago by Sama ▴ 60