Question: DNA sequencing Chromatogram analysis
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gravatar for dipankar275
3.3 years ago by
dipankar2750 wrote:

What are the criteria for the differentiate between heterozygous peak and noise in DNA sequencing chromatogram?

sequencing genome • 2.8k views
ADD COMMENTlink modified 3.3 years ago by Denise - Open Targets5.0k • written 3.3 years ago by dipankar2750
1
gravatar for Denise - Open Targets
3.3 years ago by
UK, Hinxton, EMBL-EBI
Denise - Open Targets5.0k wrote:

I'd look at the height of the peaks. When it's simply noise they tend to be small bumps, whereas hets will be distinct peaks with 'similar' height. Check Interpretation of Sequencing Chromatograms and Identification of heterozygous point mutation events in DNA sequencing chromatograms for some nice images showing the differences between hets and noise.

ADD COMMENTlink written 3.3 years ago by Denise - Open Targets5.0k

Thanks, but how much height should be consider as a noise? is there any calculation for that?

ADD REPLYlink written 3.3 years ago by dipankar2750
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