Question: 16s rRNA gene diversity analysis
0
gravatar for Arindam Ghosh
4.7 years ago by
Arindam Ghosh350
Finland
Arindam Ghosh350 wrote:

I have isolated metagenome sequences from 3 sites amplified and sequenced 16s rRNA gene by SANGER sequencing. I want to access the diversity and compare between sites. Do I need to generate OTUs individually or at once and use groups?

Cross reference: https://www.researchgate.net/post/How_to_compare_microbial_diversity_using_MOTHUR

ADD COMMENTlink modified 4.7 years ago • written 4.7 years ago by Arindam Ghosh350

What do you mean by groups? In general, you want to call OTUs with all your samples together.

ADD REPLYlink written 4.7 years ago by fanli.gcb710

This is sanger sequencing (just noticed that bit). How many sequences do you have @ag1805x?

ADD REPLYlink written 4.7 years ago by GenoMax96k
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