16s rRNA gene diversity analysis
0
0
Entering edit mode
6.0 years ago
Arindam Ghosh ▴ 450

I have isolated metagenome sequences from 3 sites amplified and sequenced 16s rRNA gene by SANGER sequencing. I want to access the diversity and compare between sites. Do I need to generate OTUs individually or at once and use groups?

Cross reference: https://www.researchgate.net/post/How_to_compare_microbial_diversity_using_MOTHUR

mothur metagenomics sequencing 16s • 1.5k views
ADD COMMENT
0
Entering edit mode

What do you mean by groups? In general, you want to call OTUs with all your samples together.

ADD REPLY
0
Entering edit mode

This is sanger sequencing (just noticed that bit). How many sequences do you have @ag1805x?

ADD REPLY

Login before adding your answer.

Traffic: 1810 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6