I am trying to identify miRNA from fastq file. I am using miRDeep2 for this purpose. As mentioned in the in the tutorial regarding the mature and precursor sequences of miRNA, I have downloaded it from miRBase version 21. While running the mirdeep2.pl command im getting this error in the report.log file.
" #Starting miRDeep2 /home/titan4/mirdeep2_0_0_8/src/miRDeep2.pl reads_collapsed.fa hg38.fa reads_collapsed_vs_genome.arf mature_new.fa none precursor_new.fa -t H.sapiens
miRDeep2 started at 12:3:54
testing input files
started: 12:3:54 sanity_check_mature_ref.pl mature_new.fa
[1;31mError: [0mproblem with mature_new.fa Error in line 5.177: The sequence
contains characters others than [acgtunACGTUN]
Please check your file for the following issues:
I. Sequences are allowed only to comprise characters [ACGTNacgtn]. II. Identifiers are not allowed to have withespaces.
Can some one help.