Question: RABT by cufflinks
0
gravatar for EpiExplorer
4.4 years ago by
EpiExplorer70
New Zealand
EpiExplorer70 wrote:

Hi,

I realised that while running cufflinks I missed an option of -g GTF-guide and I ran cufflinks with only --GTF option. What would be the difference in results of cufflinks assembly without using -g option? I wanted to do reference guided assembly.

Here is my command: cufflinks -p 8 -o /home/cufflinks --library-type fr-firststrand --GTF Genes/genes.gtf Sample1_Merged.bam

Thanks.

rna-seq • 1.5k views
ADD COMMENTlink written 4.4 years ago by EpiExplorer70
0
gravatar for EpiExplorer
4.4 years ago by
EpiExplorer70
New Zealand
EpiExplorer70 wrote:

I think I figured out the difference. The confusion was because I thought -G and -g needs to be applied together. But actually for reference guided assembly, only -g option is required. The command that I ran before would only assemble known annotations through GTF file.

Sorry for creating a new post for this silly question.

ADD COMMENTlink modified 4.4 years ago • written 4.4 years ago by EpiExplorer70

No worries, it's OK :)

ADD REPLYlink written 4.4 years ago by _r_am31k
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