I have files of data that have Many of the columns but i need sequence of DNA ,I want to convert them to fasta then convert them to fastq or convert it directly to fastq , this files reach to gigabyte, what I must do ???? This is the data we have (tsv format) we need to convert it to FastQ this is screenshot of it
For those who may happen to reach this thread by way of search in future you can convert a fasta file to fastq format using
reformat.sh from BBMap suite.
Please remember that the Q-scores created here are fake (example below sets Q-scores to 35 for all bases).
reformat.sh in=test.fa out=fake.fq qfake=35