I wanted to know How can I get individual complete gene sequences from metagenomics assembled contigs. I have done following steps:
- metagenomics assembly of a sample using Ray-Meta.
- ORF prediction doen on all contigs
- Predicted ORF has used for BLASTX
- Protein Functional and Organism annotation based top blastx hits. Now I want to get the gene sequences of top hits. For example, I am interested to get a gene sequence corresponding to amylase of Vibrio neptunius, which is the top hit of BLASTX.
Since I have done BLASTX, using the genebank ID I can get the genepept info, see the link below , but not able to get the gene sequence. http://www.ncbi.nlm.nih.gov/protein/WP_045977982
Please answer me if any have some ideas.