Question: comparing transcriptomes of two species
gravatar for victoria_aleks
2.9 years ago by
victoria_aleks30 wrote:

Hello! :) I have an idea of comparing two related but different species in order to learn if there are differences in their expression profiles. I used the bam files after tophat2 mapping in HTSeq to generate "counts-tables" and then chose top most expressed genes and compare the data. My question would be: do I need to normilize in some way the HTSeq counts (if so, then how?) or there is no sense in that since the species are different? Thanks a lot in advance!

transcriptom • 826 views
ADD COMMENTlink modified 2.9 years ago by genomax68k • written 2.9 years ago by victoria_aleks30

Did you map the two libraries to different genomes? Did you have more than one condition in each strain?

ADD REPLYlink written 2.9 years ago by Asaf5.6k
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