Adapters SureSelect QXT
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7.8 years ago
Folder40g ▴ 190

Hi

I'm analysing some exome (SureSelectQXT). I've asked people that sent me the data about the adapters, which are supposed to be CTGTCTCTTGATCAC.

I've checked this adapter in the files and I saw that about 6% of the reads contain that sequence (bot R1 and R2) followed by some other sequence.

> R1:
GAATCCATAACAGAGAAGTCCAGCATTGCAACCATGACCAGCGTGGGCAAGTCCAGGACCACCACCGAGTCCAGTGCCTGTCTCTTGATCACATCTACG
CCTGCTGGGAGGCTGCTCGCGGGAACTCAGGGAGACTGGGGATGTGTCTCTTGATCACATCTACGCACAGCTCCACCGTAAGGCGAATCTCGTATGCCGT

> R2:
GAGCCGGCTGAGCAGGAAGCGGCCGCCGTCAGGTAAGCGGCTTCGGGGGCCCGCGGGCCTGTCTCTTGATCACAAGTCTCGACGTCAGCGCGATCTAGTG
GCACTTTGGGAGGCCAAGGTGGGCGGATCACGAGGTCCTGTCTCTTGATCACAAGTCTCGACGTCAGCGCGATCTAGTGTAGATCTCGGTGGTCGCCGTA

I have asked for the whole adapter so I can remove it from those sequences, but they have no idea. In the manual page of SureSelectQXT, the only info that appears is the CTGTCTCTTGATCAC as adapter trim.

I don't thinks is a big deal, but if I can I think it would be a good idea to remove those adapters from the reads. I guess this is part of the adapter but I have no information about this.

Any clue?

SureSelect QXT exome • 2.3k views
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