Any paper or journal review about repeated multiple testing for ChIP-seq replicates?
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4.8 years ago

Dear all:

I need to understand multiple repeated testing for biological ChIP-seq replicates, to observe test variation in repeated multiple test. Can anyone tell me any related papers, journal review to explain the topic that I raised? I also prefer to look at any existing Bioconductor packages that implement multiple repeated testing for ChIP-seq replicates. Thanks a lot.

Best regards:

Jura

multiple testing ChIP-Seq paper repeated test • 957 views
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What exactly do you mean by "multiple repeated testing"? Do you just mean "multiple testing", or do you mean some sort of iterative multiple testing issue?

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Dear Devon Ryan:

Thanks for your quick respond. I am looking for paper or journal review about Multiple testing for ChIP-seq replicates. Yes, I'll be grateful if you are able to interpret difference between multiple testing and iterative multiple testing that you mentioned. Thanks a lot

Best regards:

Jurat

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Just read over the diffBind vignette for testing replicates. I'm not sure that they ever bothered writing a paper, but that's the standard tool.

Regarding some sort of iterative process, I've never heard of anyone needing to do that.

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I would take a look at this paper that came up with a method for calculating an "irreproducible discovery rate" (IDR), which they claim is analogous to the FDR but leverages replicates to quantify reproducibility. The method seems to particularly work well for ChiP-seq replicates, as that is the application they discuss. They also created the "idr" R package to calculate IDR which is found here (https://cran.r-project.org/web/packages/idr/index.html ). The method apparently works on all the replicates at once, so there is no need to iterate through each replicate and then some how combine them.

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