Displaying a virtual electrophoresis map
1
0
Entering edit mode
5.3 years ago

Hello,

I want to display a virtual electrophoresis map based on a DNA template (i.e. a long DNA sequence) and some primer pairs. I do not want to do PCR experiment and run a real gel. Do you know if there is a tool to help me?

Thanks for any comments.

Yongjie

electrophoresis map PCR • 1.5k views
ADD COMMENT
0
Entering edit mode
5.3 years ago
natasha.sernova ★ 3.9k

An old main paper:

Automated band mapping in electrophoretic gel images using background information

http://nar.oxfordjournals.org/content/33/9/2806.full

Some modern approach:

Beyond gel electrophoresis: Microfluidic separations, fluorescence burst analysis, and DNA stretching

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3595390/

Other special cases for DNA-gels.

A review of many sequencing techniques : Next Generation DNA Sequencing and the Future of Genomic Medicine

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960862/

SnapGene: Cloning is simpler when you can see exactly what you are doing.

https://www.snapgene.com/products/snapgene/about_snapgene/

VIRS: A visual tool for identifying restriction sites in multiple DNA sequences.

http://www.ncbi.nlm.nih.gov/pubmed/19637395

DNA Fragment Analysis by Capillary Electrophoresis

https://tools.thermofisher.com/content/sfs/manuals/4474504.pdf

Restriction Landmark Genomic Scanning (RLGS) spot identification by second generation virtual RLGS in multiple genomes with multiple enzyme combinations

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2235865/

BAC-HAPPY Mapping (BAP Mapping): A New and Efficient Protocol for Physical Mapping

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816996/

ADD COMMENT
0
Entering edit mode

Thanks for your answer, but my question seems not be solved. I hope to input a DNA sequence (as templates) and some primer pairs. Then,the tool can generate a virtual electrophoretic gel image, where each lane correpsonds to the amplification result of a primer pair.

ADD REPLY

Login before adding your answer.

Traffic: 2665 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6