Question: Displaying a virtual electrophoresis map
gravatar for Yongjie Zhang
3.5 years ago by
UC Berkeley, USA/ Shanxi Univ, China
Yongjie Zhang80 wrote:


I want to display a virtual electrophoresis map based on a DNA template (i.e. a long DNA sequence) and some primer pairs. I do not want to do PCR experiment and run a real gel. Do you know if there is a tool to help me?

Thanks for any comments.


pcr electrophoresis map • 1.1k views
ADD COMMENTlink modified 18 months ago by Biostar ♦♦ 20 • written 3.5 years ago by Yongjie Zhang80
gravatar for natasha.sernova
3.5 years ago by
natasha.sernova3.7k wrote:

An old main paper:

Automated band mapping in electrophoretic gel images using background information

Some modern approach:

Beyond gel electrophoresis: Microfluidic separations, fluorescence burst analysis, and DNA stretching

Other special cases for DNA-gels.

A review of many sequencing techniques : Next Generation DNA Sequencing and the Future of Genomic Medicine

SnapGene: Cloning is simpler when you can see exactly what you are doing.

VIRS: A visual tool for identifying restriction sites in multiple DNA sequences.

DNA Fragment Analysis by Capillary Electrophoresis

Restriction Landmark Genomic Scanning (RLGS) spot identification by second generation virtual RLGS in multiple genomes with multiple enzyme combinations

BAC-HAPPY Mapping (BAP Mapping): A New and Efficient Protocol for Physical Mapping

ADD COMMENTlink modified 3.5 years ago • written 3.5 years ago by natasha.sernova3.7k

Thanks for your answer, but my question seems not be solved. I hope to input a DNA sequence (as templates) and some primer pairs. Then,the tool can generate a virtual electrophoretic gel image, where each lane correpsonds to the amplification result of a primer pair.

ADD REPLYlink modified 3.5 years ago • written 3.5 years ago by Yongjie Zhang80
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1627 users visited in the last hour