HI every one, I am working on NCBI GEO data for identifying cumulative gene for stress. in the process i have came across the signal intensity values generated from MAS5.0 and GCOS. i am interested to know that is it appropriate to change the intensity values to normalized log2 value. if yes, how to covert the MAS5.0 values to log2. The below given values are example for signal values generated from MAS5.0.
ID_REF VALUE ABS_CALL AFFX-BioB-5_at 363.244 P AFFX-BioB-M_at 311.905 P AFFX-BioB-3_at 399.004 P AFFX-BioC-5_at 259.603 P