Entering edit mode
7.7 years ago
elska
•
0
I extended usual BLOSUM alphabet with my own letter. And has scores for it.
Now looking for an alignement tool that can make Global Multiple Allignement with my score matrix.
I have:
- file weight 4 mb
- 10 seqs
- max length 721226 amino acids
- avg length 367777 amino acids
Tried:
- Clustal O. It doesn't except matrix. Checked sources - didn't find ability.
- Clustal W. Works too long, mb good variant.
- MAFFT. Can't extend alphabet for it. Even if specify letter in my BLOSUM text file (unknown letter).
- MUSCLE. Memory limits. Wants to allocate 4gb of memory.
Maybe i didn't see abilities in these tools. Will be glad of any help.