Question: (Closed) EGFP under the promoter of Puromycin
0
gravatar for zahir
2.6 years ago by
zahir0
China, Guangzhou, Chinese Academy of Sciences
zahir0 wrote:

Hi All, I want to put my EGFP under the PGK promoter of Puromycin through IRES for mammalian system. I am confused whether I should delete the stop codon of puromycin and put IRES and then EGFP and stop sequences or do not remove the stop codons of puromycin. Please Let me explain how does it work. Thanks

sequence genome gene • 527 views
ADD COMMENTlink written 2.6 years ago by zahir0

Hello zahir!

We believe that this post does not fit the main topic of this site.

Not related to bioinformatics

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 2.6 years ago by WouterDeCoster37k

Try http://biology.stackexchange.com/

ADD REPLYlink written 2.6 years ago by Daniel Swan13k
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