Question: illumina results to do genotype imputation,about AB to ATCG
gravatar for sacuba1
4.1 years ago by
sacuba10 wrote:

Recently , I studied Illumina chicken 60k SNP chip. The orignal data was writed in plink map and ped file. My idea are as follows:

  1. use plink to QC
  2. convert the data after QC to vcf format
  3. use beagle4 to genotype imputation
  4. do some analysis

While in step 3, the beagle4 does not support for the AB format. I tried to use the gengen (refrence to to convert AB to ACTG. The gengen needs the snptable which has 4 columns like
Name SNP ILMN Strand Customer Strand
200003 [T/C] BOT TOP
200006 [A/G] TOP BOT
200047 [T/C] BOT TOP
200050 [C/G] TOP BOT
200052 [T/A] BOT TOP

I may got the first 3 columns but the last column data was gone. So here I want to ask you the way to got the data or the way to genotype impute without converting AB to ACTG . Look forward to your favourable reply.

ADD COMMENTlink modified 18 months ago by mw9550 • written 4.1 years ago by sacuba10

shoud i add a comment to up my post?

ADD REPLYlink written 4.1 years ago by sacuba10
gravatar for mw955
18 months ago by
mw9550 wrote:

Hey, I'm sure you've already figured this out but for anyone else reading this, check the documentation for gengen:

"one can easily generate such a file from the BeadStudio software: simply click the "SNPTable" tab after opening a project file, then use "column chooser" to select the "Name", "SNP", "ILMN Strand" and "Customer Strand" columns only, then click "export" to save the output to a file with the four columns."

Hope it helps someone, I spent the day building files for GenGen and made the mistake of skimming over the documentation. Could have saved me time if I read it the first time.

ADD COMMENTlink written 18 months ago by mw9550
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