correction for multiple testing
1
0
Entering edit mode
5.9 years ago
ashkan ▴ 130

Hi Guys,

after my analysis I have got 3000 genes and I have read counts for all of them in two condition. I want to compare two conditions. do you guys know how to get the p-values for comparison of two conditions (I have 3 columns ). I think I also have to correct for multiple testing? do you guys know how to do that?

Thanks

RNA-Seq • 2.1k views
ADD COMMENT
2
Entering edit mode
5.9 years ago

See DESeq2

ADD COMMENT
0
Entering edit mode

I am now doing Differential expression analysis. just I want to compare them statistically.

ADD REPLY
1
Entering edit mode

Following the DESeq2 vignette is by far the best way for you to apply the correct statistics. If you're asking what kind of statistical test to use, then DESeq2 implements a Wald test, with Benjamin Hochberg multiple test correction (FDR)

ADD REPLY

Login before adding your answer.

Traffic: 1220 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6