Entering edit mode
7.7 years ago
Edalat
▴
30
Hi I am doing miRNA expression analysis. the experiment was done using human model. I am finding difficulty to do annotation. The chip used is miRNA_4.0.(affymetrix). I extracted the expression dataset. what should be done?
Affymetrix annotations for 4.0 array are available on this page (scroll down to additional support).
I need csv format and the file availabe is this "miRNA 4.0 Annotations, Unsupported, CSV format (25 MB)" what is the meaning of unsupported in the middle?
My guess .. Affymetrix does not officially support it but it should work i.e. you won't be able to call Affy tech support with questions about that file.
another question,
it is maybe a ridicules question but Better to ask the way than go astray:
why the number of rows after extracting and annotation in miRNA array expression analysis do not change but in small RNA-seq analysis change?
I don't know. Is it possible that miRNA array's have unique spots so you get one row per miRNA?
I don`t know, there is 36249 rows before and after of annotation,In fact, I do not see annotation effect
excuse me,is it possible in a small RNA-seq experiment ,2 samples need adapter trimming and 2 samples do not need?
My understanding is small RNAseq experiment should need trimming, unless the other two samples were run for a short number of cycles (or if the adapters have been pre-trimmed).