Hi~ I recently use hisat2 for RNA-seq analysis. First, I execute 'hisat2-build' for generating index with the reference about exonsites and splice sites(hisat2-build --s splicesite.txt --exon exonsites.txt), but it alway can't accomplish. The hisat2 would warn up with "is not reverse-deterministic, so reverse-determinize...", and occupied a lot of memory, the maximum is 400G. the program generated eight .ht2 files ,I don't know if the files are complete.I need help...
Question: hisat2-build failed with 'is not reverse-deterministic, so reverse-determinize'2
3.2 years ago by
ranzhou1005 • 0
ranzhou1005 • 0 wrote:
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