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• Statistical testing for differential expression in DESeq
  I am a bit confused about the test DESeq uses to call differentially expressed genes. I realize t...
• What is the actual cause of excessive zeroes in single cell RNA-seq data? Is it PCR?
  First, sorry if I am missing something basic - I am a programmer recently turned bioinformatician...
• The purpose of scaling to a negative binomial distribution in single cell RNA-seq
  Hi, I'm still a bit new to computational analysis of single cell data, but I'm doing my best to ...
• RPKM and differential expression analysis
  Hello all, I am trying to analyse differential expression in a dataset for which I only have RPK...
• Modeling the NB distribution for differential expression
  Hi everyone, Apologies in advance if this is a repeat post. I couldn't quite find the answer I ...
• What Makes One Probability Distribution Better For Rna-Seq Than Another?
  Many experts claim that the negative binomial distribution is better than the Poisson distributio...
• multivariate analysis of RNA-seq
  When you have only one variable with two categories (e.g. disease vs control) to compare for RNA-...
• Why number of reads in a sample that are assigned to gene i can be modeled by a negative bionomial distribution ?
  Hi,   I'm reading the paper called: Differential expression analysis for sequence count data. i...