hi all, just wondering if anyone has used sanger sequencing to brige/close the gap between two contigs that have been generated as a result of 454-newbler for prokaryotic genomes?
i.e. when contig reordering programs are used to tile 454 contigs against reference genomes, they tend to push many contigs (genomic regions with repeats, horizontally acquired, unique genes) to the end. to map the location of these contigs to their correct position, has anyone used genomic dna as template and carried out sanger sequencing with primers going outward?
I hope I have not written this question too confusingly. thanks !