I have a list of sgRNA that were designed for CRISPR experiment and their corresponding gene (symbol).
I'm trying to compute a percent peptide score, that is the location of these guides along their target.
I need to find a way to align all these sequences along their target and output the location of the alignment as well as the size of the gene.
I though about using a shell script along with an alignment tool but I need help in getting it started.
Thank you in advance.