Hello everyone!
Complete noob to bioinformatics, have no idea what I'm doing (all I've done programming-wise in my life is finish Python Codeacademy) -- hoping someone here can help me out!
So basically, from my understanding, the Conbind online tool (http://www.conbind.org/) will take a gene you give it, look up homologs from the species you tell it, and then align the two genes based off transcription binding site motifs. I've conducted a multiple sequence alignment of a human gene and its mouse homolog using MAFFT.
Basically, what I'd like to do, since I already have the multiple sequence alignment (and the formatting for Conbind's alignment is not what my boss wants), is find a way to feed my MSA to Conbind, thereby allowing it to skip the alignment process, and have it just search for motifs in the already-aligned sequences I feed it.
Now, the Conbind website has some very basic documentation on its API -- http://www.conbind.org/api. It specifies that someone should have experience with REST architecture before trying to do anything new with Conbind. My questions are as follows:
- Does what I'm trying to do even sound feasible in terms of the documentation they give and REST APIs in general?
- Anyone have any experience with what I'm trying to accomplish?
- If no one can help me out directly, can y'all at least point me in the right direction of resources that would allow me to do this?
ANY help would be much appreciated! Thanks!