I get 16sRNA sequencing from illumina MiSeq. I Run fastQC and I get the following quality plot.
Why the first 6 position has the same quality. Is it the barcode not removed ?
And when I look into my fastq , the header specify a sequence : TAATGCGCCCTATCCT . is it the barcode too ? And why they are 17 nucleotides ?
@HWI-D00473:173:H3KJTBCXX:2:1101:11770:2101 1:N:0:TAATGCGCCCTATCCT CCGTCAATTCATTTAAGTTTCATACTTGCGTACGTACTCCCCAGGCGGATTACTTATCGCGTTAGCTTGGGCGCTGAGGTTCGACCCCCAACACCTAGTAATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCG CGCTTTAGCGTCAGTATCTGTCCAGTAAGCTGGCTTCCCCATCGGCATTCCTACAAATACCTACGACTTTCACCTCTACCCTTGTAGTTCCGCTTACCTCTCCAGTACTCTAGTCATCCAGTTTCCAACGCAATACCGAGT