Entering edit mode
7.6 years ago
zizigolu
★
4.3k
hi,
I was indentifying miRNAs by mirdeep2, for two fastq files I got result by for the third nothing :( :( :( how possible
I built index
bowtie-build genome.fa genome
I used mapper
[izadi@lbox200 bin]$ mapper.pl 3.fq -e -h -m -v -p af -s 3.fa -t 3.arf
parsing fastq to fasta format
discarding short reads
collapsing reads
mapping reads to genome index
trimming unmapped nts in the 3' ends
Log file for this run is in mapper_logs and called mapper.log_31816
Mapping statistics
#desc total mapped unmapped %mapped %unmapped
total: 2906658 704337 2202321 0.242 0.758
seq: 2906658 704337 2202321 0.242 0.758
but when quantifying nothing happened
quantifier.pl -p hairpin.fa -m mature.fa -r sample.fa
getting samples and corresponding read numbers
Converting input files
building bowtie index
mapping mature sequences against index
mapping read sequences against index
Mapping statistics
**No mapping file given**
how possible I got result for two samples but for third I donno what happened
any suggestion...