Any packages for normalization by read depth of the 75%tile gene?
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7.6 years ago
cfarmeri ▴ 210

Hi, Biostars.

I want to normalize read count data (by STAR) by read depth of the 75%tile gene like this paper.

Anyone knows useful packages (R, python ,etc) ?

(I tried by "scale" function in R, but couldn't do this.)

thanks!!

RNA-Seq next-gen • 1.6k views
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Hi, You can try "quantile" function to calculate 75-percentile value. This link might help you: http://www.r-tutor.com/elementary-statistics/numerical-measures/percentile .

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