Hello, I wanted to do single cell RNA seq on a very rare cytokine population. Which means I will have to do intracellular staining and then sort the cytokine producing cells. Inorder to detect cytokines, I will have to fix my cells and I read the fixing kills the cells and there is no RNA. is there a way to do single cell RNA seq on fixed cells ?? My single cell RNa seq system is by a company called Fluidgm (C1 system). Thanks !
You should ask this on seqanswers, as it is slightly off-topic for Biostars.