Entering edit mode
7.6 years ago
Mehmet
▴
820
Dear All:
I have a proteome file generated from Next Gen. Sequencing. I want to search my proteome file against to blast database using command line version. How to do that? Please do not send me manual of blast command line. I know little about Linux. I need a clear explanation of command line usage.
Thank you.
That is difficult without a clear explanation of what kind data you have and what you want to do, lacking issues:
Hi: I have used trinity for de novo transcriptome assembly, I have generated it. Later, I have used Transdecoder to translate transcripts into amino acids. Now I have proteome file of the transcripts. My point is that I want to see my proteome file ( each protein sequence) matches to which protein in NCBI database.
If you have a proteome, you can use makeblastdb to make a database out of your translated proteins and search NCBI with each of the proteins from your database with blastp or blastx (whatever you prefer).