Question: differential gene expression analysis
0
gravatar for rajasekargutha
3.3 years ago by
India
rajasekargutha30 wrote:

i performed Rankprod for Differential gene expression analysis b/w normal and test samples of zebrafish, and i got up regulated and down regulated genes. but i found some genes are commonly present in both up and down regulated genes. how it possible that there are commonly present????????/

rna-seq next-gen genome • 1.2k views
ADD COMMENTlink modified 3.3 years ago • written 3.3 years ago by rajasekargutha30
1

Were you testing at the transcript level? How else could you find the same gene going both ways in the same dataset?

BTW, unless you have a LOT of samples, you're strongly encouraged NOT to use rankprod.

ADD REPLYlink written 3.3 years ago by Devon Ryan94k

I am working on expression array samples downloaded from ncgi geo and I have 45 test samples and 38 control samples

ADD REPLYlink written 3.3 years ago by rajasekargutha30
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