Question: differential gene expression analysis
gravatar for rajasekargutha
4.1 years ago by
rajasekargutha40 wrote:

i performed Rankprod for Differential gene expression analysis b/w normal and test samples of zebrafish, and i got up regulated and down regulated genes. but i found some genes are commonly present in both up and down regulated genes. how it possible that there are commonly present????????/

rna-seq next-gen genome • 1.3k views
ADD COMMENTlink modified 4.1 years ago • written 4.1 years ago by rajasekargutha40

Were you testing at the transcript level? How else could you find the same gene going both ways in the same dataset?

BTW, unless you have a LOT of samples, you're strongly encouraged NOT to use rankprod.

ADD REPLYlink written 4.1 years ago by Devon Ryan97k

I am working on expression array samples downloaded from ncgi geo and I have 45 test samples and 38 control samples

ADD REPLYlink written 4.1 years ago by rajasekargutha40
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