Question: RNAseq differential expression analysis
0
gravatar for monikakumar2017
2.1 years ago by
monikakumar20170 wrote:

Hi. i am new to transcriptome analysis , i wanted to see the differential expression of some genes. I have got RNAseq of single reads and i have aligned my transcript reads to the genome with bowtie tool but how shall i proceed? Thank you

rna-seq • 775 views
ADD COMMENTlink modified 2.0 years ago by Biostar ♦♦ 20 • written 2.1 years ago by monikakumar20170
3

May be you should read some tutorials:

Informatics of RNA-Seq.

ADD REPLYlink written 2.1 years ago by geek_y8.8k

Ok. Good. Thank you a lot

ADD REPLYlink written 2.1 years ago by monikakumar20170

If you have more specific questions after reading some tutorials and papers in which they perform something similar as you want to do feel free to ask them. But currently your question is rather vague and you should be able to find plenty of information online.

ADD REPLYlink written 2.1 years ago by WouterDeCoster35k
1

Which organism are you working on? Unless it's prokaryotic, bowtie is probably not appropriate.

ADD REPLYlink written 2.1 years ago by WouterDeCoster35k

I am working on Vibrio cholerae

ADD REPLYlink written 2.1 years ago by monikakumar20170
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