RNAseq differential expression analysis

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7.5 years ago

monikakumar2017 • 0

Hi. i am new to transcriptome analysis , i wanted to see the differential expression of some genes. I have got RNAseq of single reads and i have aligned my transcript reads to the genome with bowtie tool but how shall i proceed? Thank you

RNA-Seq • 1.6k views

ADD COMMENT • link updated 7.4 years ago by Biostar 20 • written 7.5 years ago by monikakumar2017 • 0

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May be you should read some tutorials:

Informatics of RNA-Seq.

ADD REPLY • link 7.5 years ago by GouthamAtla 12k

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Ok. Good. Thank you a lot

ADD REPLY • link 7.5 years ago by monikakumar2017 • 0

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If you have more specific questions after reading some tutorials and papers in which they perform something similar as you want to do feel free to ask them. But currently your question is rather vague and you should be able to find plenty of information online.

ADD REPLY • link 7.5 years ago by WouterDeCoster 47k

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Which organism are you working on? Unless it's prokaryotic, bowtie is probably not appropriate.