Allele-specific quantification methods for short tandem repeats?
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5.0 years ago
michealsmith ▴ 770

Hi, I would like to get very accurate quantification of the amount of different alleles of short tandem repeats. For example in DNA pool, at one locus, there could be 20% CA, 50% CACA, 30% CACACA, so what could I do?I don't think general SYBR green qPCR could work

microsatellite pcr qpcr allele • 999 views
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