Question: Must I Have The .Sff Files In Order To Run Mira3
gravatar for Walter
8.9 years ago by
Walter150 wrote:

I have only the fasta files and qual files and i need to run mira3. is it posibble to run mira3 and where can i obtain the .sff files.

mira • 1.7k views
ADD COMMENTlink modified 8.4 years ago by iw9oel_ad6.0k • written 8.9 years ago by Walter150
gravatar for iw9oel_ad
8.9 years ago by
iw9oel_ad6.0k wrote:

No, you do not require SFF files. If you have SFF files, the first step is to convert to Fasta, qual and traceinfo files. As you have Fasta and qual files, you should be able to skip this step. However, you may need a traceinfo file (or equivalent) to inform mira3 about parameters such as strain name and library insert sizes. The manual contains details of how to make a traceinfo file by hand, if you do not have one.

ADD COMMENTlink written 8.9 years ago by iw9oel_ad6.0k

It specifically mentions on the feature page that it will accept FASTA and FASTQ input formats:

ADD REPLYlink written 8.9 years ago by Daniel Swan13k
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