I am starting to play with open source variant callers such as - MuTect, SomaticSniper, Strelka, Varscan2. All those algorithms need always - normal.bam and tumor.bam. My question is, where can i get normal.bam?? I need to put one normal (non-cancer) patient in my sequencing run? Or can I download normal.bam from Internet and using it in my variant calling?
Thank you for sharing your experiences with variant calling in somatic mode.