How can I explain an insertion of 158 bp in intronic region suppress the gene expression??
I have one resistant and one susceptible line, I sequenced a gene in both and found 158bp insertion in intronic region in the susceptible line, where the gene expression is significantly lower compared to resistant line. How can I explain this?
For note: I have sequenced 1kb promoter region upstream of ATG but wouldn't see any sequence varaition