Hi everyone, I think i have problems when I import data in partek. I have mouse affy arrays. Lets say 4 diets in triplicates. I imported all data together with the RMA setting and did analysis for DEG and got nothing! Since i have very strong evidence from other experiments that it definitely has an effect I cant believe that there are no DEGs. So then I imported 1 diet at a time withRMA setting each time. Then I merged these together into one filr and checked for DEG. I got 1500 now. But I am not sure if I can rely on that. Strangely when i look back at the intensities for some genes these replicates have ditto value for e.g. If value is 6.3020 for a probe thrn it is 6.3020 in my other replicates of the same diet. How can the intensities be like this? Effect of RMA? Data imported wrongly? Thanks in advance!
Question: Importing data onto partek
3.4 years ago by
sshibin • 0
sshibin • 0 wrote:
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