I did de novo transcriptome assembly using paired-end reads from Illumina Hiseq 2000 for a non-model organism. I mapped back reads to transcriptome assembly. If I'm not wrong, the average of mapping coverage shows the read number (in average) that mapped to each reconstructed transcript (contig). Could you please let me know if this number is important and why? and how I can calculate it?
Thank you for your answer