Question: What does the reads per sequences mean
0
gravatar for arronar
2.5 years ago by
arronar160
Austria
arronar160 wrote:

Hi. I'm reading a paper and try to understand a plot of it. In the x-axis there is the position from promoter (bp) and in the y-axis it writes reads per 10^6 sequences.

What exactly that "reads per 10^6 sequences" means?

rna-seq • 696 views
ADD COMMENTlink written 2.5 years ago by arronar160

That sounds indeed rather cryptic, perhaps you can add from which article this plot is?

ADD REPLYlink written 2.5 years ago by WouterDeCoster38k

here is the DOI of the article : doi:10.1038/nature09652

ADD REPLYlink written 2.5 years ago by arronar160

Oh, that looks like normalized read count to me. Like FPKM but without taking transcript length into account (presumably not required for this).

ADD REPLYlink written 2.5 years ago by WouterDeCoster38k

Oh. thank you very much.

ADD REPLYlink written 2.5 years ago by arronar160
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